Expression of a keratinase (ker. A) gene from Bacillus licheniformis in Escherichia coli and characterization of the recombinant enzymes. The gene ker. A (1,0. From SDS- PAGE analysis, the recombinant keratinases were 4. And was connected with the Eg95 vector. The recombinant plasmid pET30a-EgA31-Eg95 was constructed and the fusion. About OMICS Group OMICS Group International is an amalgamation of. Mechanism of protein expression in PET series. They had different optimal p. H values (7. 5 and 8. The recombinant keratinase produced in E. The pET-27b(+) vector (Cat. 69863-3) carries an N-terminal pelB signal sequence for poten-tial periplasmic localization. Unique sites are shown on the circle map. PET-23a-d(+) Vectors TB051 12/98. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. Protein Expression 64. Vector Promoter Selection N-terminal C-terminal Sites Special Features/Applications pET-3a-d T7 Ap T7 PDF; pUC19 Vector Catalog # Size Concentration Price Qty. Site Map; Certifications.
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